Purpose: The aim of this study was to evaluate the effect of different training amplitudes on the autonomic nervous system (ANS) responses and recovery through heart rate (HR), heart rate variability (HRV) and rate of perceived effort (RPE). Methods: In a counterbalanced design, male trained cyclists (24.8 ± 6.9 years old) performed three training sessions matched by total duration (20 min) and by mean power (55% of maximal power output, ), but with different effort:pause ratio and different amplitudes. Continuous training (CT) was composed by efforts of 55% of maximal power (Pmax). The low amplitude training (LAT) was composed by efforts with 80% of Pmax and pauses of 30% of Pmax, and high amplitude training (HAT) with efforts of 110% of Pmax and passive pauses (0% of Pmax). Data were analyzed using a two-way ANOVA with repeated measures or non parametric correspondent. Results: HAT promoted superior RPE (9.0 ± 1.0 au) in comparison to the LAT (3.8 ± 2.8 au), and CT (2.8 ± 1.5 au) with p <.01, and higher increments in the maximal HR (172.8 +/-11.8 bpm) in comparison to the CT (140.8 ± 14.2 bpm, p = .001). Regarding HRV, the three protocols had similar results, except by the CT, which did not return to baseline levels after 24h of rest. Conclusions: The HAT showed higher impact on the RPE and in maximum HR at the end of the session and the HRV variables showed similar responses despite the difference in the training protocols.
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